ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human Granzyme B

3486-4APW-P1-1

Publications: 1

Documents

Questions?

A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb	MT8610, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus
Plate	Pre-coated ELISpot plate, white MSIP (mAb MT28)
Peptide pools	SARS-CoV-2 (S1), scanning
	SARS-CoV-2 (SNMO), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human granzyme B in response to SARS-CoV-2 (S1 scan+SNMO) peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.

Recommendation

Product details

Product	ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human Granzyme B
Application	ELISpot
Analyte	Granzyme B
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC (250,000 cells/well) from two convalescent individuals, previously confirmed COVID-19 positive, were incubated 48 h in the absence or presence of the SARS-CoV-2 S1 scanning pool and SARS-CoV-2 S N M O defined peptide pool in the presence of anti-CD28 mAb. The number of cells secreting Granzyme B was analyzed by ELISpot and counted using MabtechIRIS.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Product highlights

Highlighted research, Published May 17, 2022

Third vaccine dose important for T cell response against Omicron

Three doses mRNA vaccine had better T cell responses than recipients of two doses. Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B were used to assess T cell immunity in mRNA vaccine recipients.

Highlighted research, Published December 16, 2021

SARS-CoV-2-specific immune responses in patients with autoimmune diseases

Patients with rheumatic diseases showed T cell responses comparable to healthy control. Patients with MS and after mRNA vaccination show different memory B cell IgG responses. SARS-CoV-2 specific immune responses in autoimmunity was analyzed using FluoroSpot, ELISpot Path, and Mabtech IRIS.

Highlighted research, Published December 2, 2021

Importance of studying SARS-CoV-2-specific memory B cells

When studying antibodies in circulation, remember that memory B cells might hide out of sight. In this study, detection of SARS-CoV-2-specific T and memory B cells 6 and 15 months after infection.

Highlighted research, Published November 24, 2021

Polyfunctional T cells with Mabtech IRIS

Study of development and persistence of virus-specific cellular and humoral immune responses, during the acute phase and two follow-ups after COVID-19 disease in hospitalized patients. Mabtech’s FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α were analyzed with Mabtech IRIS.

Highlighted research, Published June 30, 2020

T cell immunity in COVID-19

Much emphasis has been put on antibody responses, but also T cells are important in the context of COVID-19. In this study, the authors characterized SARS-CoV-2-specific T cell responses by using an extensive FACS panel, peptide stimulations, tetramer staining, and Mabtech IFN-γ ELISpot.

Highlighted research, Published May 30, 2020

B cell FluoroSpot for sensitive ASC detection

Mabtech IRIS and IgG/IgA/IgM FluoroSpot were used to study antibody-secreting plasma cells (ASCs) specific for the nucleocapsid (N) protein. FluoroSpot was shown to be more sensitive than e.g., ELISA.

Highlighted research, Published May 11, 2020

Longevity of immunity against SARS-CoV-2

Investigation of specific T cell responses to SARS-CoV 11 years after outbreak using Mabtech human IFN-γ ELISpot.

Publications (1)

Loading publications...

Analyte information

Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3002